Located on the third floor of the Main Library, holds numerous editions and rare works of incunabla, medieval manuscripts, early modern works, and many other treasures in bibliographic history. It most prominently has a rich Shakespeare collection that include all four folios, a set of Pavier quartos (1619), a copy of the 1640 Poems, and the stationer's fragment (ca. 1603–1607) that documents the possible lost play by Shakespeare, Love's Labor’s Won. The RBML also holds 16th- and 17th-century editions of works by John Northbrooke, Thomas Kyd's Spanish Tragedy, Thomas Heywood, William Prynne's Histro-mastix, among many others.

The Library offers access to the full productions of the 37 BBC Shakespeare The Library offers access to the full productions of the 37 BBC Shakespeare Plays. The plays are available in streaming video, unabridged and with closed captions. Follow this link to view the plays in the online catalog.

A 27-vol. set. Each volume includes a comprehensive overview of scholarship on a different play. Follow this link to view the volumes in the online catalog.